RNA
Part:BBa_K3944046
Designed by: Olof Dahlman Group: iGEM21_Chalmers-Gothenburg (2021-09-30)
FAS1 antisense
Antisense RNA sequences designed to inhibit expression of S.Cerevisiae native fatty acid synthase (FAS) system, to be combined with the introduction of bacterial FAS2 system. Down-regulating it with anti-sense RNA ensures that the cell maintains some crucial production of fatty acids for growth.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 19
Illegal PstI site found at 50 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 19
Illegal PstI site found at 50 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 19
Illegal PstI site found at 50 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 19
Illegal PstI site found at 50 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |